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Page 20 Cell Reports 44, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/page+20+cell+reports+44/pHAGE-BRAF-N486D+(Plasmid+%23116186)/pm40844875-668-221-216
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Magnetic Beads:Article Title: TCF25 serves as a nutrient sensor to orchestrate metabolic adaptation and cell death by enhancing lysosomal acidification under glucose starvation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-TCF25 ABclonal Cat# A15140; RRID:AB_2762027 anti-ATP6V1A Abcam Cat# ab199326; RRID:AB_2802119 anti-ATP6V0D1 Abcam Cat# ab56441; RRID:AB_940402 anti-CTSB CST Cat# 31718; RRID:AB_2687580 anti-CTSD Proteintech Cat# 21327-1-AP; RRID:AB_10733646 anti-Cleaved Caspase-3 CST Cat# 9664; RRID:AB_2070042 anti-Caspase-3 ABclonal Cat# A2156; RRID:AB_2862975 anti-phospho-MLKL Abcam Cat# ab196436; RRID:AB_2687465 anti-MLKL ABclonal Cat# A5579; RRID:AB_2766355 anti-FLAG ABclonal Cat# AE004; RRID:AB_2771921 anti-HA ABclonal Cat# AE008; RRID:AB_2770404 anti-c-Myc SANTA CRUZ Cat# sc-40; RRID:AB_627268 anti-LAMP1 CST Cat# 3243; RRID:AB_2134478 anti-COX IV ZEN-BIOSCIENCE Cat# 200147; RRID:AB_2722715 anti-Lamin A/C ZEN-BIOSCIENCE Cat# 201015-5D12 anti-Tubulin Abbkine Cat# A01030 anti-LC3 CST Cat# 12741; RRID:AB_2617131 anti-NCOA4 Santa Cruz Cat# sc-373739; RRID:AB_10915585 anti-FTH1 HUABIO Cat# ET1610-78; AB_2923223 anti-SLC7A11 CST Cat# 12691; RRID:AB_2687474 anti-SLC3A2 ABclonal Cat# A5702; RRID:AB_2766461 GAPDH HUABIO Cat# ET1601-4; RRID: AB_2923224 Anti-Rab7 CST Cat# 9367T; RRID:AB_1904103 Chemicals, peptides, and recombinant proteins Bafilomycin A1 MedChemExpress Cat#HY-100558 Smac mimetic MedChemExpress Cat#HY-15989 Z-VAD-FMK MedChemExpress Cat#HY-16658B H2DCFDA MedChemExpress Cat#HY-D0940 Nitroxoline MedChemExpress Cat#HY-B1159 CA-074 methyl ester MedChemExpress Cat#HY-100350 BAY-876 MedChemExpress Cat#HY-100017 Deferoxamine MedChemExpress Cat#HY-B1625 Deferasirox MedChemExpress Cat#HY-17359 Ouabain MedChemExpress Cat#HY-B0542 Concanamycin A MedChemExpress Cat#HY-N1724 Recombinant mouse TNF-α R&D System Cat#410-MT-025/CF Lipo293F TM transfection reagent Beyotime Cat#C0518 Lipo8000 TM transfection reagent Beyotime Cat#C0533 LysoTracker green Beyotime Cat#C1047S Mito-Tracker Green Beyotime Cat#C1048 LysoSensor Yeasen Cat#40767ES50 FerroOrange DOJINDO Cat#F374 (Continued on next page) Cell Reports 44, 116186, September 23, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER pH-sensitive FITC Thermofisher Cat#D-7172 pH-insensitive TMR Thermofisher Cat#D-1819 Pyruvate BasalMedia Cat#S410JV Glucose-6-phosphate BBI Life Sciences Cat#620045 protein A/G- magnetic beads MedChemExpress Cat# HY-K0202 Cathepsin B Magic Red (MR) substrates Abcam Cat#ab270772 AlexaFluor 488-dextran (10 kDa) Thermofisher Cat#D22910 Critical commercial assays CellTiter-Lumi TM Assay kit Beyotime Cat#C0065 Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat#P0027 Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Duolink® In Situ Red Starter Kit Sigma Cat#DUO92101 Lysosome Enrichment Kit Thermofisher Cat#89839 Intracellular pH Calibration Buffer Kit Invitrogen Cat#P35379 ADP-Glo Kinase Assay Promega Cat#V6930 Aspartate aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C010-2-1 Alanine aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C009-2-1 Experimental models: cell lines Mouse dermal fibroblast (MDF) This paper N/A Mouse lung fibroblasts (MIF) This paper N/A Adipocytes This paper N/A Bone marrow-derived macrophages (BMDM) This paper N/A Hela ATCC Cat#CRM-CCL-2 HEK293T ATCC Cat#CRL-11268 HT-29 ATCC Cat#HTB-38 SKOV3 ATCC Cat#HTB-77 786-O ATCC Cat#CRL-1932 HCT8 ATCC Cat#CCL-244 DLD1 ATCC Cat#CCL-221 A549 ATCC Cat#CRM-CCL-185 Experimental models: organisms/strains C57BL/6J Gempharmatech N/A TCF25 − /− BRL Medicine Inc N/A Recombinant DNA pLVX-HA-hsATP6V1A This paper N/A pLVX-HA-hsATP6V0d1 This paper N/A pLVX-HA-musATP6V0d1 This paper N/A pLVX-FLAG-musTCF25 This paper N/A pLVX-mcherry-hsTCF25 This paper N/A pLVX-mcherry-musTCF25 This paper N/A PCMV3-FLAG-hsTCF25 This paper N/A PCDH-EF1-GAL3-GFP This paper N/A RFP-GFP-LC3 This paper N/A pLJM1-Tmem192-mRFP-3xHA 53 Addgene Cat#134631 (Continued on next Protein Extraction:Article Title: TCF25 serves as a nutrient sensor to orchestrate metabolic adaptation and cell death by enhancing lysosomal acidification under glucose starvation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-TCF25 ABclonal Cat# A15140; RRID:AB_2762027 anti-ATP6V1A Abcam Cat# ab199326; RRID:AB_2802119 anti-ATP6V0D1 Abcam Cat# ab56441; RRID:AB_940402 anti-CTSB CST Cat# 31718; RRID:AB_2687580 anti-CTSD Proteintech Cat# 21327-1-AP; RRID:AB_10733646 anti-Cleaved Caspase-3 CST Cat# 9664; RRID:AB_2070042 anti-Caspase-3 ABclonal Cat# A2156; RRID:AB_2862975 anti-phospho-MLKL Abcam Cat# ab196436; RRID:AB_2687465 anti-MLKL ABclonal Cat# A5579; RRID:AB_2766355 anti-FLAG ABclonal Cat# AE004; RRID:AB_2771921 anti-HA ABclonal Cat# AE008; RRID:AB_2770404 anti-c-Myc SANTA CRUZ Cat# sc-40; RRID:AB_627268 anti-LAMP1 CST Cat# 3243; RRID:AB_2134478 anti-COX IV ZEN-BIOSCIENCE Cat# 200147; RRID:AB_2722715 anti-Lamin A/C ZEN-BIOSCIENCE Cat# 201015-5D12 anti-Tubulin Abbkine Cat# A01030 anti-LC3 CST Cat# 12741; RRID:AB_2617131 anti-NCOA4 Santa Cruz Cat# sc-373739; RRID:AB_10915585 anti-FTH1 HUABIO Cat# ET1610-78; AB_2923223 anti-SLC7A11 CST Cat# 12691; RRID:AB_2687474 anti-SLC3A2 ABclonal Cat# A5702; RRID:AB_2766461 GAPDH HUABIO Cat# ET1601-4; RRID: AB_2923224 Anti-Rab7 CST Cat# 9367T; RRID:AB_1904103 Chemicals, peptides, and recombinant proteins Bafilomycin A1 MedChemExpress Cat#HY-100558 Smac mimetic MedChemExpress Cat#HY-15989 Z-VAD-FMK MedChemExpress Cat#HY-16658B H2DCFDA MedChemExpress Cat#HY-D0940 Nitroxoline MedChemExpress Cat#HY-B1159 CA-074 methyl ester MedChemExpress Cat#HY-100350 BAY-876 MedChemExpress Cat#HY-100017 Deferoxamine MedChemExpress Cat#HY-B1625 Deferasirox MedChemExpress Cat#HY-17359 Ouabain MedChemExpress Cat#HY-B0542 Concanamycin A MedChemExpress Cat#HY-N1724 Recombinant mouse TNF-α R&D System Cat#410-MT-025/CF Lipo293F TM transfection reagent Beyotime Cat#C0518 Lipo8000 TM transfection reagent Beyotime Cat#C0533 LysoTracker green Beyotime Cat#C1047S Mito-Tracker Green Beyotime Cat#C1048 LysoSensor Yeasen Cat#40767ES50 FerroOrange DOJINDO Cat#F374 (Continued on next page) Cell Reports 44, 116186, September 23, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER pH-sensitive FITC Thermofisher Cat#D-7172 pH-insensitive TMR Thermofisher Cat#D-1819 Pyruvate BasalMedia Cat#S410JV Glucose-6-phosphate BBI Life Sciences Cat#620045 protein A/G- magnetic beads MedChemExpress Cat# HY-K0202 Cathepsin B Magic Red (MR) substrates Abcam Cat#ab270772 AlexaFluor 488-dextran (10 kDa) Thermofisher Cat#D22910 Critical commercial assays CellTiter-Lumi TM Assay kit Beyotime Cat#C0065 Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat#P0027 Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Duolink® In Situ Red Starter Kit Sigma Cat#DUO92101 Lysosome Enrichment Kit Thermofisher Cat#89839 Intracellular pH Calibration Buffer Kit Invitrogen Cat#P35379 ADP-Glo Kinase Assay Promega Cat#V6930 Aspartate aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C010-2-1 Alanine aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C009-2-1 Experimental models: cell lines Mouse dermal fibroblast (MDF) This paper N/A Mouse lung fibroblasts (MIF) This paper N/A Adipocytes This paper N/A Bone marrow-derived macrophages (BMDM) This paper N/A Hela ATCC Cat#CRM-CCL-2 HEK293T ATCC Cat#CRL-11268 HT-29 ATCC Cat#HTB-38 SKOV3 ATCC Cat#HTB-77 786-O ATCC Cat#CRL-1932 HCT8 ATCC Cat#CCL-244 DLD1 ATCC Cat#CCL-221 A549 ATCC Cat#CRM-CCL-185 Experimental models: organisms/strains C57BL/6J Gempharmatech N/A TCF25 − /− BRL Medicine Inc N/A Recombinant DNA pLVX-HA-hsATP6V1A This paper N/A pLVX-HA-hsATP6V0d1 This paper N/A pLVX-HA-musATP6V0d1 This paper N/A pLVX-FLAG-musTCF25 This paper N/A pLVX-mcherry-hsTCF25 This paper N/A pLVX-mcherry-musTCF25 This paper N/A PCMV3-FLAG-hsTCF25 This paper N/A PCDH-EF1-GAL3-GFP This paper N/A RFP-GFP-LC3 This paper N/A pLJM1-Tmem192-mRFP-3xHA 53 Addgene Cat#134631 (Continued on next Membrane:Article Title: TCF25 serves as a nutrient sensor to orchestrate metabolic adaptation and cell death by enhancing lysosomal acidification under glucose starvation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-TCF25 ABclonal Cat# A15140; RRID:AB_2762027 anti-ATP6V1A Abcam Cat# ab199326; RRID:AB_2802119 anti-ATP6V0D1 Abcam Cat# ab56441; RRID:AB_940402 anti-CTSB CST Cat# 31718; RRID:AB_2687580 anti-CTSD Proteintech Cat# 21327-1-AP; RRID:AB_10733646 anti-Cleaved Caspase-3 CST Cat# 9664; RRID:AB_2070042 anti-Caspase-3 ABclonal Cat# A2156; RRID:AB_2862975 anti-phospho-MLKL Abcam Cat# ab196436; RRID:AB_2687465 anti-MLKL ABclonal Cat# A5579; RRID:AB_2766355 anti-FLAG ABclonal Cat# AE004; RRID:AB_2771921 anti-HA ABclonal Cat# AE008; RRID:AB_2770404 anti-c-Myc SANTA CRUZ Cat# sc-40; RRID:AB_627268 anti-LAMP1 CST Cat# 3243; RRID:AB_2134478 anti-COX IV ZEN-BIOSCIENCE Cat# 200147; RRID:AB_2722715 anti-Lamin A/C ZEN-BIOSCIENCE Cat# 201015-5D12 anti-Tubulin Abbkine Cat# A01030 anti-LC3 CST Cat# 12741; RRID:AB_2617131 anti-NCOA4 Santa Cruz Cat# sc-373739; RRID:AB_10915585 anti-FTH1 HUABIO Cat# ET1610-78; AB_2923223 anti-SLC7A11 CST Cat# 12691; RRID:AB_2687474 anti-SLC3A2 ABclonal Cat# A5702; RRID:AB_2766461 GAPDH HUABIO Cat# ET1601-4; RRID: AB_2923224 Anti-Rab7 CST Cat# 9367T; RRID:AB_1904103 Chemicals, peptides, and recombinant proteins Bafilomycin A1 MedChemExpress Cat#HY-100558 Smac mimetic MedChemExpress Cat#HY-15989 Z-VAD-FMK MedChemExpress Cat#HY-16658B H2DCFDA MedChemExpress Cat#HY-D0940 Nitroxoline MedChemExpress Cat#HY-B1159 CA-074 methyl ester MedChemExpress Cat#HY-100350 BAY-876 MedChemExpress Cat#HY-100017 Deferoxamine MedChemExpress Cat#HY-B1625 Deferasirox MedChemExpress Cat#HY-17359 Ouabain MedChemExpress Cat#HY-B0542 Concanamycin A MedChemExpress Cat#HY-N1724 Recombinant mouse TNF-α R&D System Cat#410-MT-025/CF Lipo293F TM transfection reagent Beyotime Cat#C0518 Lipo8000 TM transfection reagent Beyotime Cat#C0533 LysoTracker green Beyotime Cat#C1047S Mito-Tracker Green Beyotime Cat#C1048 LysoSensor Yeasen Cat#40767ES50 FerroOrange DOJINDO Cat#F374 (Continued on next page) Cell Reports 44, 116186, September 23, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER pH-sensitive FITC Thermofisher Cat#D-7172 pH-insensitive TMR Thermofisher Cat#D-1819 Pyruvate BasalMedia Cat#S410JV Glucose-6-phosphate BBI Life Sciences Cat#620045 protein A/G- magnetic beads MedChemExpress Cat# HY-K0202 Cathepsin B Magic Red (MR) substrates Abcam Cat#ab270772 AlexaFluor 488-dextran (10 kDa) Thermofisher Cat#D22910 Critical commercial assays CellTiter-Lumi TM Assay kit Beyotime Cat#C0065 Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat#P0027 Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Duolink® In Situ Red Starter Kit Sigma Cat#DUO92101 Lysosome Enrichment Kit Thermofisher Cat#89839 Intracellular pH Calibration Buffer Kit Invitrogen Cat#P35379 ADP-Glo Kinase Assay Promega Cat#V6930 Aspartate aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C010-2-1 Alanine aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C009-2-1 Experimental models: cell lines Mouse dermal fibroblast (MDF) This paper N/A Mouse lung fibroblasts (MIF) This paper N/A Adipocytes This paper N/A Bone marrow-derived macrophages (BMDM) This paper N/A Hela ATCC Cat#CRM-CCL-2 HEK293T ATCC Cat#CRL-11268 HT-29 ATCC Cat#HTB-38 SKOV3 ATCC Cat#HTB-77 786-O ATCC Cat#CRL-1932 HCT8 ATCC Cat#CCL-244 DLD1 ATCC Cat#CCL-221 A549 ATCC Cat#CRM-CCL-185 Experimental models: organisms/strains C57BL/6J Gempharmatech N/A TCF25 − /− BRL Medicine Inc N/A Recombinant DNA pLVX-HA-hsATP6V1A This paper N/A pLVX-HA-hsATP6V0d1 This paper N/A pLVX-HA-musATP6V0d1 This paper N/A pLVX-FLAG-musTCF25 This paper N/A pLVX-mcherry-hsTCF25 This paper N/A pLVX-mcherry-musTCF25 This paper N/A PCMV3-FLAG-hsTCF25 This paper N/A PCDH-EF1-GAL3-GFP This paper N/A RFP-GFP-LC3 This paper N/A pLJM1-Tmem192-mRFP-3xHA 53 Addgene Cat#134631 (Continued on next In Situ:Article Title: TCF25 serves as a nutrient sensor to orchestrate metabolic adaptation and cell death by enhancing lysosomal acidification under glucose starvation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-TCF25 ABclonal Cat# A15140; RRID:AB_2762027 anti-ATP6V1A Abcam Cat# ab199326; RRID:AB_2802119 anti-ATP6V0D1 Abcam Cat# ab56441; RRID:AB_940402 anti-CTSB CST Cat# 31718; RRID:AB_2687580 anti-CTSD Proteintech Cat# 21327-1-AP; RRID:AB_10733646 anti-Cleaved Caspase-3 CST Cat# 9664; RRID:AB_2070042 anti-Caspase-3 ABclonal Cat# A2156; RRID:AB_2862975 anti-phospho-MLKL Abcam Cat# ab196436; RRID:AB_2687465 anti-MLKL ABclonal Cat# A5579; RRID:AB_2766355 anti-FLAG ABclonal Cat# AE004; RRID:AB_2771921 anti-HA ABclonal Cat# AE008; RRID:AB_2770404 anti-c-Myc SANTA CRUZ Cat# sc-40; RRID:AB_627268 anti-LAMP1 CST Cat# 3243; RRID:AB_2134478 anti-COX IV ZEN-BIOSCIENCE Cat# 200147; RRID:AB_2722715 anti-Lamin A/C ZEN-BIOSCIENCE Cat# 201015-5D12 anti-Tubulin Abbkine Cat# A01030 anti-LC3 CST Cat# 12741; RRID:AB_2617131 anti-NCOA4 Santa Cruz Cat# sc-373739; RRID:AB_10915585 anti-FTH1 HUABIO Cat# ET1610-78; AB_2923223 anti-SLC7A11 CST Cat# 12691; RRID:AB_2687474 anti-SLC3A2 ABclonal Cat# A5702; RRID:AB_2766461 GAPDH HUABIO Cat# ET1601-4; RRID: AB_2923224 Anti-Rab7 CST Cat# 9367T; RRID:AB_1904103 Chemicals, peptides, and recombinant proteins Bafilomycin A1 MedChemExpress Cat#HY-100558 Smac mimetic MedChemExpress Cat#HY-15989 Z-VAD-FMK MedChemExpress Cat#HY-16658B H2DCFDA MedChemExpress Cat#HY-D0940 Nitroxoline MedChemExpress Cat#HY-B1159 CA-074 methyl ester MedChemExpress Cat#HY-100350 BAY-876 MedChemExpress Cat#HY-100017 Deferoxamine MedChemExpress Cat#HY-B1625 Deferasirox MedChemExpress Cat#HY-17359 Ouabain MedChemExpress Cat#HY-B0542 Concanamycin A MedChemExpress Cat#HY-N1724 Recombinant mouse TNF-α R&D System Cat#410-MT-025/CF Lipo293F TM transfection reagent Beyotime Cat#C0518 Lipo8000 TM transfection reagent Beyotime Cat#C0533 LysoTracker green Beyotime Cat#C1047S Mito-Tracker Green Beyotime Cat#C1048 LysoSensor Yeasen Cat#40767ES50 FerroOrange DOJINDO Cat#F374 (Continued on next page) Cell Reports 44, 116186, September 23, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER pH-sensitive FITC Thermofisher Cat#D-7172 pH-insensitive TMR Thermofisher Cat#D-1819 Pyruvate BasalMedia Cat#S410JV Glucose-6-phosphate BBI Life Sciences Cat#620045 protein A/G- magnetic beads MedChemExpress Cat# HY-K0202 Cathepsin B Magic Red (MR) substrates Abcam Cat#ab270772 AlexaFluor 488-dextran (10 kDa) Thermofisher Cat#D22910 Critical commercial assays CellTiter-Lumi TM Assay kit Beyotime Cat#C0065 Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat#P0027 Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Duolink® In Situ Red Starter Kit Sigma Cat#DUO92101 Lysosome Enrichment Kit Thermofisher Cat#89839 Intracellular pH Calibration Buffer Kit Invitrogen Cat#P35379 ADP-Glo Kinase Assay Promega Cat#V6930 Aspartate aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C010-2-1 Alanine aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C009-2-1 Experimental models: cell lines Mouse dermal fibroblast (MDF) This paper N/A Mouse lung fibroblasts (MIF) This paper N/A Adipocytes This paper N/A Bone marrow-derived macrophages (BMDM) This paper N/A Hela ATCC Cat#CRM-CCL-2 HEK293T ATCC Cat#CRL-11268 HT-29 ATCC Cat#HTB-38 SKOV3 ATCC Cat#HTB-77 786-O ATCC Cat#CRL-1932 HCT8 ATCC Cat#CCL-244 DLD1 ATCC Cat#CCL-221 A549 ATCC Cat#CRM-CCL-185 Experimental models: organisms/strains C57BL/6J Gempharmatech N/A TCF25 − /− BRL Medicine Inc N/A Recombinant DNA pLVX-HA-hsATP6V1A This paper N/A pLVX-HA-hsATP6V0d1 This paper N/A pLVX-HA-musATP6V0d1 This paper N/A pLVX-FLAG-musTCF25 This paper N/A pLVX-mcherry-hsTCF25 This paper N/A pLVX-mcherry-musTCF25 This paper N/A PCMV3-FLAG-hsTCF25 This paper N/A PCDH-EF1-GAL3-GFP This paper N/A RFP-GFP-LC3 This paper N/A pLJM1-Tmem192-mRFP-3xHA 53 Addgene Cat#134631 (Continued on next Kinase Assay:Article Title: TCF25 serves as a nutrient sensor to orchestrate metabolic adaptation and cell death by enhancing lysosomal acidification under glucose starvation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-TCF25 ABclonal Cat# A15140; RRID:AB_2762027 anti-ATP6V1A Abcam Cat# ab199326; RRID:AB_2802119 anti-ATP6V0D1 Abcam Cat# ab56441; RRID:AB_940402 anti-CTSB CST Cat# 31718; RRID:AB_2687580 anti-CTSD Proteintech Cat# 21327-1-AP; RRID:AB_10733646 anti-Cleaved Caspase-3 CST Cat# 9664; RRID:AB_2070042 anti-Caspase-3 ABclonal Cat# A2156; RRID:AB_2862975 anti-phospho-MLKL Abcam Cat# ab196436; RRID:AB_2687465 anti-MLKL ABclonal Cat# A5579; RRID:AB_2766355 anti-FLAG ABclonal Cat# AE004; RRID:AB_2771921 anti-HA ABclonal Cat# AE008; RRID:AB_2770404 anti-c-Myc SANTA CRUZ Cat# sc-40; RRID:AB_627268 anti-LAMP1 CST Cat# 3243; RRID:AB_2134478 anti-COX IV ZEN-BIOSCIENCE Cat# 200147; RRID:AB_2722715 anti-Lamin A/C ZEN-BIOSCIENCE Cat# 201015-5D12 anti-Tubulin Abbkine Cat# A01030 anti-LC3 CST Cat# 12741; RRID:AB_2617131 anti-NCOA4 Santa Cruz Cat# sc-373739; RRID:AB_10915585 anti-FTH1 HUABIO Cat# ET1610-78; AB_2923223 anti-SLC7A11 CST Cat# 12691; RRID:AB_2687474 anti-SLC3A2 ABclonal Cat# A5702; RRID:AB_2766461 GAPDH HUABIO Cat# ET1601-4; RRID: AB_2923224 Anti-Rab7 CST Cat# 9367T; RRID:AB_1904103 Chemicals, peptides, and recombinant proteins Bafilomycin A1 MedChemExpress Cat#HY-100558 Smac mimetic MedChemExpress Cat#HY-15989 Z-VAD-FMK MedChemExpress Cat#HY-16658B H2DCFDA MedChemExpress Cat#HY-D0940 Nitroxoline MedChemExpress Cat#HY-B1159 CA-074 methyl ester MedChemExpress Cat#HY-100350 BAY-876 MedChemExpress Cat#HY-100017 Deferoxamine MedChemExpress Cat#HY-B1625 Deferasirox MedChemExpress Cat#HY-17359 Ouabain MedChemExpress Cat#HY-B0542 Concanamycin A MedChemExpress Cat#HY-N1724 Recombinant mouse TNF-α R&D System Cat#410-MT-025/CF Lipo293F TM transfection reagent Beyotime Cat#C0518 Lipo8000 TM transfection reagent Beyotime Cat#C0533 LysoTracker green Beyotime Cat#C1047S Mito-Tracker Green Beyotime Cat#C1048 LysoSensor Yeasen Cat#40767ES50 FerroOrange DOJINDO Cat#F374 (Continued on next page) Cell Reports 44, 116186, September 23, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER pH-sensitive FITC Thermofisher Cat#D-7172 pH-insensitive TMR Thermofisher Cat#D-1819 Pyruvate BasalMedia Cat#S410JV Glucose-6-phosphate BBI Life Sciences Cat#620045 protein A/G- magnetic beads MedChemExpress Cat# HY-K0202 Cathepsin B Magic Red (MR) substrates Abcam Cat#ab270772 AlexaFluor 488-dextran (10 kDa) Thermofisher Cat#D22910 Critical commercial assays CellTiter-Lumi TM Assay kit Beyotime Cat#C0065 Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat#P0027 Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Duolink® In Situ Red Starter Kit Sigma Cat#DUO92101 Lysosome Enrichment Kit Thermofisher Cat#89839 Intracellular pH Calibration Buffer Kit Invitrogen Cat#P35379 ADP-Glo Kinase Assay Promega Cat#V6930 Aspartate aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C010-2-1 Alanine aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C009-2-1 Experimental models: cell lines Mouse dermal fibroblast (MDF) This paper N/A Mouse lung fibroblasts (MIF) This paper N/A Adipocytes This paper N/A Bone marrow-derived macrophages (BMDM) This paper N/A Hela ATCC Cat#CRM-CCL-2 HEK293T ATCC Cat#CRL-11268 HT-29 ATCC Cat#HTB-38 SKOV3 ATCC Cat#HTB-77 786-O ATCC Cat#CRL-1932 HCT8 ATCC Cat#CCL-244 DLD1 ATCC Cat#CCL-221 A549 ATCC Cat#CRM-CCL-185 Experimental models: organisms/strains C57BL/6J Gempharmatech N/A TCF25 − /− BRL Medicine Inc N/A Recombinant DNA pLVX-HA-hsATP6V1A This paper N/A pLVX-HA-hsATP6V0d1 This paper N/A pLVX-HA-musATP6V0d1 This paper N/A pLVX-FLAG-musTCF25 This paper N/A pLVX-mcherry-hsTCF25 This paper N/A pLVX-mcherry-musTCF25 This paper N/A PCMV3-FLAG-hsTCF25 This paper N/A PCDH-EF1-GAL3-GFP This paper N/A RFP-GFP-LC3 This paper N/A pLJM1-Tmem192-mRFP-3xHA 53 Addgene Cat#134631 (Continued on next Recombinant:Article Title: TCF25 serves as a nutrient sensor to orchestrate metabolic adaptation and cell death by enhancing lysosomal acidification under glucose starvation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-TCF25 ABclonal Cat# A15140; RRID:AB_2762027 anti-ATP6V1A Abcam Cat# ab199326; RRID:AB_2802119 anti-ATP6V0D1 Abcam Cat# ab56441; RRID:AB_940402 anti-CTSB CST Cat# 31718; RRID:AB_2687580 anti-CTSD Proteintech Cat# 21327-1-AP; RRID:AB_10733646 anti-Cleaved Caspase-3 CST Cat# 9664; RRID:AB_2070042 anti-Caspase-3 ABclonal Cat# A2156; RRID:AB_2862975 anti-phospho-MLKL Abcam Cat# ab196436; RRID:AB_2687465 anti-MLKL ABclonal Cat# A5579; RRID:AB_2766355 anti-FLAG ABclonal Cat# AE004; RRID:AB_2771921 anti-HA ABclonal Cat# AE008; RRID:AB_2770404 anti-c-Myc SANTA CRUZ Cat# sc-40; RRID:AB_627268 anti-LAMP1 CST Cat# 3243; RRID:AB_2134478 anti-COX IV ZEN-BIOSCIENCE Cat# 200147; RRID:AB_2722715 anti-Lamin A/C ZEN-BIOSCIENCE Cat# 201015-5D12 anti-Tubulin Abbkine Cat# A01030 anti-LC3 CST Cat# 12741; RRID:AB_2617131 anti-NCOA4 Santa Cruz Cat# sc-373739; RRID:AB_10915585 anti-FTH1 HUABIO Cat# ET1610-78; AB_2923223 anti-SLC7A11 CST Cat# 12691; RRID:AB_2687474 anti-SLC3A2 ABclonal Cat# A5702; RRID:AB_2766461 GAPDH HUABIO Cat# ET1601-4; RRID: AB_2923224 Anti-Rab7 CST Cat# 9367T; RRID:AB_1904103 Chemicals, peptides, and recombinant proteins Bafilomycin A1 MedChemExpress Cat#HY-100558 Smac mimetic MedChemExpress Cat#HY-15989 Z-VAD-FMK MedChemExpress Cat#HY-16658B H2DCFDA MedChemExpress Cat#HY-D0940 Nitroxoline MedChemExpress Cat#HY-B1159 CA-074 methyl ester MedChemExpress Cat#HY-100350 BAY-876 MedChemExpress Cat#HY-100017 Deferoxamine MedChemExpress Cat#HY-B1625 Deferasirox MedChemExpress Cat#HY-17359 Ouabain MedChemExpress Cat#HY-B0542 Concanamycin A MedChemExpress Cat#HY-N1724 Recombinant mouse TNF-α R&D System Cat#410-MT-025/CF Lipo293F TM transfection reagent Beyotime Cat#C0518 Lipo8000 TM transfection reagent Beyotime Cat#C0533 LysoTracker green Beyotime Cat#C1047S Mito-Tracker Green Beyotime Cat#C1048 LysoSensor Yeasen Cat#40767ES50 FerroOrange DOJINDO Cat#F374 (Continued on next page) Cell Reports 44, 116186, September 23, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER pH-sensitive FITC Thermofisher Cat#D-7172 pH-insensitive TMR Thermofisher Cat#D-1819 Pyruvate BasalMedia Cat#S410JV Glucose-6-phosphate BBI Life Sciences Cat#620045 protein A/G- magnetic beads MedChemExpress Cat# HY-K0202 Cathepsin B Magic Red (MR) substrates Abcam Cat#ab270772 AlexaFluor 488-dextran (10 kDa) Thermofisher Cat#D22910 Critical commercial assays CellTiter-Lumi TM Assay kit Beyotime Cat#C0065 Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat#P0027 Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Duolink® In Situ Red Starter Kit Sigma Cat#DUO92101 Lysosome Enrichment Kit Thermofisher Cat#89839 Intracellular pH Calibration Buffer Kit Invitrogen Cat#P35379 ADP-Glo Kinase Assay Promega Cat#V6930 Aspartate aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C010-2-1 Alanine aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C009-2-1 Experimental models: cell lines Mouse dermal fibroblast (MDF) This paper N/A Mouse lung fibroblasts (MIF) This paper N/A Adipocytes This paper N/A Bone marrow-derived macrophages (BMDM) This paper N/A Hela ATCC Cat#CRM-CCL-2 HEK293T ATCC Cat#CRL-11268 HT-29 ATCC Cat#HTB-38 SKOV3 ATCC Cat#HTB-77 786-O ATCC Cat#CRL-1932 HCT8 ATCC Cat#CCL-244 DLD1 ATCC Cat#CCL-221 A549 ATCC Cat#CRM-CCL-185 Experimental models: organisms/strains C57BL/6J Gempharmatech N/A TCF25 − /− BRL Medicine Inc N/A Recombinant DNA pLVX-HA-hsATP6V1A This paper N/A pLVX-HA-hsATP6V0d1 This paper N/A pLVX-HA-musATP6V0d1 This paper N/A pLVX-FLAG-musTCF25 This paper N/A pLVX-mcherry-hsTCF25 This paper N/A pLVX-mcherry-musTCF25 This paper N/A PCMV3-FLAG-hsTCF25 This paper N/A PCDH-EF1-GAL3-GFP This paper N/A RFP-GFP-LC3 This paper N/A pLJM1-Tmem192-mRFP-3xHA 53 Addgene Cat#134631 (Continued on next |